RT-qPCR Kits often provide different results depending on primer selection – the new UltraMarathonRT RT-qPCR Kit solves this challenge
, /PRNewswire-PRWeb/ — RNAConnect, developer of enzymatic tools for visualizing and manipulating RNA, today announced the release of the UltraMarathonRT® Two-Step RT-qPCR Kit, a next-generation tool for RNA researchers conducting gene expression analysis.
The UltraMarathonRT® RT-qPCR Kit dramatically improves data reliability in quantitative PCR by maintaining consistent cycle threshold (Ct) values, regardless of RT primer type, RNA complexity or amplicon location. This innovation empowers researchers to confidently quantify RNA across a broad spectrum of applications, including biomarker validation, high GC content RNA analysis, transcriptomic studies, and more.
UltraMarathonRT (uMRT) was designed to overcome the challenges of complex RNA secondary structures and low RNA inputs. Because uMRT provides full 5′-3′ RNA transcript coverage, PCR amplicon location along a first-strand cDNA molecule synthesized by uMRT does not affect qPCR quantification. This greatly reduces the potential for …